Isolation of extracellular cobalt-free corrinoid from Methanosarcina barkeri.
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چکیده
منابع مشابه
Isolation of subunits from Methanosarcina barkeri ATPase: nucleotide-binding site in the alpha subunit.
The alpha (62,000-dalton) and beta (49,000-dalton) subunits of Methanosarcina barkeri ATPase were purified to homogeneity. The subunits and ATPase complex were trypsinized in the presence of various nucleotides. ATP and ADP changed the trypsin sensitivity of the alpha subunit in the complex and isolated forms, suggesting the presence of a nucleotide-binding site in the alpha subunit.
متن کاملBioenergetics of methanogenesis from acetate by Methanosarcina barkeri.
Methane formation from acetate by resting cells of Methanosarcina barkeri was accompanied by an increase in the intracellular ATP content from 0.9 to 4.0 nmol/mg of protein. Correspondingly, the proton motive force increased to a steady-state level of -120 mV. The transmembrane pH gradient however, was reversed under these conditions and amounted to +20 mV. The addition of the protonophore 3,5,...
متن کاملDifferential in vitro methylation and synthesis of the 480-kilodalton corrinoid protein in Methanosarcina barkeri grown on different substrates.
The 480-kDa corrinoid protein was significantly methylated in extracts of acetate- but not methanol-grown cells incubated with 14CH3OH, in part because of its decreased synthesis in cells grown on substrates other than acetate. In addition, a 200-kDa corrinoid protein was methylated in extracts of methanol- but not acetate-grown cells.
متن کاملParamagnetic centers of carbon monoxide dehydrogenase from aceticlastic Methanosarcina barkeri.
Carbon monoxide dehydrogenase from Methanosarcina barkeri, purified to 95% homogeneity, contains 30 Fe, 2 Ni, 1 Zn, and 1 Cu (per alpha 2 beta 2 enzyme). Core extrusion experiments indicate 6 [4Fe-4S] clusters/tetramer, and electron paramagnetic resonance (epr) spectroscopy detects at least one of these clusters, in the reduced form, with apparent g values of 2.05, 1.94, and 1.90, and Em9.2-390...
متن کاملMethanogenic cleavage of acetate by lysates of Methanosarcina barkeri.
Cell lysates of acetate-grown Methanosarcina barkeri 227 were found to cleave acetate to CH4 and CO2. The aceticlastic reaction was identified by using radioactive methyl-labeled acetate. Cell lysates decarboxylated acetate in a nitrogen atmosphere, conserving the methyl group in methane. The rate of methanogenesis from acetate in the cell lysates was comparable to that observed with whole cell...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1989
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.53.2455